BriClone Hybridoma Cloning Supplement

BriClone was first developed in 1994 by the DCU research team to improve the efficiency of cloning medium used in the post-fusion stages of hybridoma production and for improving the efficiency of hybridoma cell cloning. At the request of the wider research community, this high quality supplement was made more widely available and has been in continuous production for over 25 years.

The use of feeder cells to support the outgrowth of hybridomas has many disadvantages, such as batch-to-batch variation of the feeder cells, and competition between feeder cells and freshly-fused hybridomas for nutrients in the culture medium. BriClone has been specially developed by the NICB’s dedicated team to overcome these common problems in the post-fusion period.

BriClone is produced and distributed from our state-of-the-art facility at the NICB, Dublin City University, and is distributed frozen in 100ml bottles as a concentrate for dilution in basal medium.

BriClone is available to purchase through a global network of cell culture reagent distributors. Please email us on and we will connect you with a high-quality, trusted distributor in your local region and answer any technical questions you may have.

Increased Efficiency in Hybridoma Production
No Time Consuming Preparations
Elimination of Batch Variation
No Depletion of Nutrients by Feeder Cells
Very Competitively Priced
Screened for Mycoplasma Contamination

Briclone Information Sheets